Thursday, July 14, 2005

Week 5

We prepared ourselves for the p450 experiment by making 1 mol of KPO4, NADPH, G6PO4, G6PDH, and a 1:4 dilution of MgCl. Then we made our tubes in preparation for the experiment. We then set up charts of ‘time dependent’ and ‘protein dependent’ experiments. Today we proceeded to complete the experiment doing the time dependent after a lecture of the procedure. We only had time to run the timed experiment, so Dr. Clarke ran the protein experiment over night. We did a binding experiment with Dr. Harp. We talked about IC50 and how we were going to set up the experiment. Then we talked about how to set up the experiment with the tubes and the different concentrations of drug. Since we were not able to finish our own drugs, we used some that Dr. Harp already had for us. Then we proceeded to interpret the data from Dr. Harp’s experiment since we did not finish. Today Dr. Clarke told us that we were to do another experiment like the one from Monday and Tuesday, but on a smaller scale. We will be using different concentrations of DPP and of our individual drugs (Phenacetin, Dextromethorphan, ibuprofen, and Terpenadine). This experiment will tell us if DPP is an inhibiter of the drugs.

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