Week 4 with Dr. Harp
We preformed two tlc’s to determine our product but no such luck. So we had to use a column chromatography to determine if we had any product in out solution. Column Chromatography is just like tlc just that the produce travels downward due to gravity. Using tubes we collected samples of the solution to run tlc’s on the individual tubes. Today we flushed our column chromatography solution and rotervaped our completed DFB and prepared an NMR test solution. We learned about how we use an NMR to determine the products magnetic field and from that we can see what is in our solution. We went to wake forest to se a 300 mhz magnet that was used for the NMR. Then we went back to PTCRC to heir a lecture that Dr. Harp had to give. Today we helped clean the lab up and organize it a little. Then we proceeded to rotervap our flush and tube solution from the other day. Once completed, we prepared solutions for the rat dissection. Dr. Harp showed us how to kill the rat properly and the important parts of the dissection.
posted by alejandro rios @ Monday, July 11, 2005
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