Week 3 with Dr. Clarke and Harp
We ran the Bradford protein assay again because the results were not reliable. Then we used the Genios machine to test the concentration of the enzymes. Last we compared and analyzed our data with one another. The nex day we helped Dr. Harp with children from a summer program making aspirin using salicylic acid and phosphoric acid as well. Then heated and filtered using filter paper. It was fun working with those kids. We helped Dr. Harp with the same kids,the next day, to separate the caffeine from tea. First we made the tea. Then we used dichloromethane and sodium hydroxide and magnesium sulfate to separate the caffeine and water from the tea. Then they scraped the caffeine and put it in to some vials and took them home. I've never work with kids that we that young and so many of them at the same time. With Doctor Clarke, we talked about Terfenadine, Dextromethorphan, S-ibuprofen, and Phenacetin. After making enough of each chemical for everyone, we picked who would do which and we poured some into vials. The amounts varied because we needed one mole of each and that is dependent on the molecular weight. Then we ran out of time so we stopped there to pick up at a later time.
posted by alejandro rios @ Monday, July 04, 2005
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